The pathway of metabolic shift regulatory system of yeast Saccharomyces cerevisiae in Fig. 6 is repeated as independent validation by randomly reshuffling the time order of microarray experiment but with the same choices of target and regulatory genes. Obviously, the proposed pathway in Fig. 6 is destroyed by the reshuffling of experimental data. The related genes are represented as ovals with different colors of light blue (fermentation), light yellow (gluconeogenesis), and gray (other relevant genes not as target genes). There are three types of lines with colors of red (activation), blue (repression), and black. In addition to the black line representing the signal pipes from genes, the red lines are shown depending on the degree of activation whilst the blue lines are shown depending on the degree of repression. The black square symbol attached on the lines is the bifurcate node from the same signal source. And the circle symbol attached on the lines is the collecting nodes from different signal sources. The colored degree bar between activation and repression is also shown in the bottom of the figure.