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Table 3 Magnitude of propagated error at different steps of data processing

From: A standard curve based method for relative real time PCR data processing

SD in crossing points

CV in crossing points

CV in non-normalized values

CV in normalized results

0.1

0.5%

7%

10%

0.2

1.0%

14%

20%

0.3

1.5%

22%

31%

0.4

2.0%

28%

40%

0.6

3.0%

45%

66%

  1. In all instances mean values are 20 in crossing points, 10 in non-normalized values and 1 in final results. See Figures 5 and 13 for more details.