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Table 3 Magnitude of propagated error at different steps of data processing

From: A standard curve based method for relative real time PCR data processing

SD in crossing points CV in crossing points CV in non-normalized values CV in normalized results
0.1 0.5% 7% 10%
0.2 1.0% 14% 20%
0.3 1.5% 22% 31%
0.4 2.0% 28% 40%
0.6 3.0% 45% 66%
  1. In all instances mean values are 20 in crossing points, 10 in non-normalized values and 1 in final results. See Figures 5 and 13 for more details.