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Figure 2 | BMC Bioinformatics

Figure 2

From: 'PACLIMS': A component LIM system for high-throughput functional genomic analysis

Figure 2

A schematic overview of the flow in information and materials managed by PACLIMS. Module 1: entry point for PACLIMS, accessed by scanning or entering a plate or plant rack's barcode; the user is directed to the appropriate web form. Modules 2 and 3: displays web forms for data entry on a new, or "parent" plate, including fields for information on mutants in each of the 24 wells. Module 4: provides access to a web form for entry of growth rate data that is collected from the parent plate and allows the user to create "copies" of the parent plate for assaying other phenotypes. Module 5: guides and documents the transfer of paper disks from four 24-well "parent" plates into the four quadrants of three 96-well plates, used for permanent storage and shipping, the latter process being recorded with Module 6. Module 7: records the revival of cultures from permanent storage, specifically the reversion of the 96-well format into four 24-well plates for pathogenicity assays. Module 8: directs the inoculation process for each 24-well plate of spores, beginning with entering a barcode for a rack of plants to be inoculated, and culminating in entering pathological data for each mutant isolate. Module 9: records phenotypic data via four different web forms, each of which records specific phenotypes; module 1 controls the particular data entry form that is accessed.

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