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Figure 7 | BMC Bioinformatics

Figure 7

From: Microarray scanner calibration curves: characteristics and implications

Figure 7

Simulation of the characteristics of dye-swap replicates. A: Dye bias is simulated by a difference in θ of the Sigmoid function; B: Dye-swap pairing; C: log mRNA concentrations for the two samples ("Sample" and "Ref"); D: Scatter plot of log intensities for one replicate of the dye-swap pair; E: Calibration curve for Cy5 ("labeled" with "Ref"); F: Calibration curve for Cy3 ("labeled" with "Sample"); G: log intensity correlation for the same sample ("Sample") labeled with two dyes in the dye-swap pair; H: MA plot for "Ref" labeled with Cy5 and "Sample" labeled with Cy3; I: MA plot for "Ref" labeled with Cy3 and "Sample" labeled with Cy5; J: log ratio correlation of the dye-swap pair. Spots colored in green are of lower average intensity, whereas spots colored in blue are of higher average intensity.

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