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Figure 1 | BMC Bioinformatics

Figure 1

From: CLPM: A Cross-Linked Peptide Mapping Algorithm for Mass Spectrometric Analysis

Figure 1

General strategy of mass spectrometric analysis of protein-protein interactions. (A) Incubate the crosslinker with the target proteins to acquire crosslinked mixtures. (B) Purify the crosslinked species by SDS-PAGE and (C) extract spots and perform in-gel, protease digestion to yield a peptide pool, which includes crosslinked and uncrosslinked peptides. (D) The peptides are submitted to mass spectrometry to acquire either MS or MS/MS data. (E) Data are subsequently analyzed.

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