From: Evaluation of absolute quantitation by nonlinear regression in probe-based real-time PCR
Model change | Mean R2 change | Intra-assay variation | Bias |
---|---|---|---|
Backgr. corrected data | -0.0065 | +27% | +15% |
Log10 transformation | 0 | +1% | -4% |
Correction of baseline drift (linear) | +0.0001 | +20% | -7% |
Correction of baseline drift (Log10) | +0.0004 | +35% | +12% |
Weight early cycles (RAW) | -0.0006 | +3% | +40% |
Weight early cycles (Backgr. corrected) | +0.0056 | +11% | +64% |
Weight late cycles | -0.0006 | +7% | +18% |