Exemplary trimer weights calculated by TICO. Positional weight matrix (PWM) values resulting from our algorithm for four exemplary trimers in the flanking regions of the TIS. Position 0 denotes the translation start. Selected trimers correspond to the most frequent subwords in the putative SD motifs determined by MED-Start  for P. aeruginosa (CCTGG, GCGCC, GCCTG, CGCCG and CGGCG). Negative weights indicate that trimer occurrences at the corresponding positions are untypical for strong TIS candidates.