Table 7 RT-PCR primers. Primers for the genes used in Real-Time RT-PCR and the length of the PCR product. Each gene is represented with the Affymetrix probe number and the Arabidopsis Genome Initiative (AGI) code.
probe | AGI | length (bp) | Forward primer | Reverse primer |
|---|---|---|---|---|
Genes common for all microarray methods | ||||
245098_at | At2g40940 | 148 | 5'-CGGAACTCAGAGGAACCATT-3' | 5'-GCAGATACCAAGCCTGATGA-3' |
245264_at | At4g17245 | 50 | 5'-CAAGACGGTGACACCTTACG-3' | 5'-TGGAATCCATGTTTGCATCT-3' |
247486_at | At5g62140 | 158 | 5'-ACGGAGGAATCGATAGGAGA-3' | 5'-TGTAGATCGGCGAGACACTC-3' |
247954_at | At5g56870 | 96 | 5'-CAGAGAGTTCCGGTGTGAGA-3' | 5'-TTCCTGCTGGTGTAGCAAAC-3' |
249125_at | At5g43450 | 99 | 5'-GTTCTTGAGCGTGGAGCATA-3' | 5'-ACCGTGGAATTTGGAGAAAG-3' |
250598_at | At5g07690 | 75 | 5'-AACAAGCGTTGATGAAGACG-3' | 5'-AAATCGGAATGGTCAAGGAG-3' |
250911_at | At5g03730 | 67 | 5'-CGAGATGAGCCGTCTAATGA-3' | 5'-TAGCAAGCTCCCACAAGATG-3' |
251058_at | At5g01790 | 133 | 5'-AGAAACTGTCGGCTTCATCA-3' | 5'-TCAGCAGAAGAGTCGAAGGA-3' |
251373_at | At3g60530 | 129 | 5'-ACCGCTTGGACCTAAAACAC-3' | 5'-TTCCGATGAGAGTTCGAATG-3' |
253302_at | At4g33660 | 83 | 5'-ACCACCACGAAAAGTTGGTT-3' | 5'-GGTCACAGCAACATTCATCC-3' |
254371_at | At4g21760 | 51 | 5'-TTCATCTTCCAGCACAGAGC-3' | 5'-TTGTTTCAGGCACCAATCAT-3' |
258181_at | At3g21670 | 96 | 5'-GCTTACGTTGGACAGCTTGA-3' | 5'-TCCCATCGATATCGTGCTTA-3' |
258468_at | At3g06070 | 100 | 5'-GTTCCTTTATCCCCAAGCAA-3' | 5'-GCATGATGAAAGGTGATGCT-3' |
259982_at | At1g76410 | 142 | 5'-TGGCTTGGATCACACTCTTC-3' | 5'-CAGGACCATCTTCACGTTGT-3' |
263653_at | At1g04310 | 83 | 5'-ACGCTTGCCAAAACATTGTA-3' | 5'-TGAGACGCTTTTCACCAAAC-3' |
264624_at | At1g08930 | 135 | 5'-TGGAATGCATCAGGAATGTT-3' | 5'-TTGCACAGAGTTGTTGAGCA-3' |
265194_at | At1g05010 | 59 | 5'-TATAATCCGGGAAGCGACTC-3' | 5'-GCTTCTTTTCCGATCAGCTC-3' |
266884_at | At2g44790 | 152 | 5'-ACTCCTACCACACCGGAATC-3' | 5'-ATCGAGACTCCCACCAAAAC-3' |
RMA specific genes | ||||
262870_at | At1g64710 | 129 | 5'-CCGATGGAAAGACCAGATTC-3' | 5'-GAAACAGAGGGTCCACCTTG-3' |
260181_at | At1g70710 | 107 | 5'-ACACAAAGCCTCGAGGAAAC-3' | 5'-CATGCTTATTGTCCCAACCA-3' |
246389_at | At1g77380 | 61 | 5'-AATGTACATCGCGCAGAAGA-3' | 5'-GACTTGAAGGCAAACCCATC-3' |
263461_at | At2g31800 | 52 | 5'-GGGACCTTGGGAGCTATCTT-3' | 5'-ACTTTGGCTGGAGAAAGACG-3' |
263891_at | At2g36850 | 139 | 5'-ATGGCTACTCGTGGGTTGTT-3' | 5'-CCAAGGACACATGCAAACAT-3' |
267592_at | At2g39710 | 128 | 5'-CGTATTGCATATCCGGTTCA-3' | 5'-CCGGTCGAAATAAGGTAACG-3' |
252278_at | At3g49530 | 73 | 5'-CGTGACCGGTTTTGTGTTTA-3' | 5'-TACTGCCGCCCTAAAGAGTC-3' |
250892_at | At5g03760 | 77 | 5'-CTGCTTGTGGACTCTCATGG-3' | 5'-TTTGATCGTTGGATCAGTGG-3' |
249876_at | At5g23060 | 103 | 5'-GCACAACAGACGTCAAAAGC-3' | 5'-CAGCAGCAACAACAATGACA-3' |
249822_at | At5g23710 | 52 | 5'-CGGGAAAAAGTTCGGTAATG-3' | 5'-AATCGAAGCAAAAGCTCCAG-3' |