Significantly different subsystems between Sargasso Sea and SEED datasets. (A). Each subsystem was bootstrapped with between 10 and 400 samples per bootstrap, and subsystems that are significantly different with 99% and 2,000 bootstraps are highlighted. Those subsystems that are significantly more prevalent in the SEED database are colored blue, and those subsystems that are significantly more prevalent in the Sargasso Sea dataset are colored red. (B). Magnified view of several different subsystems. Subsystems from amino acid synthesis, carbohydrate utilization, cofactor synthesis, fatty acids, nucleotide synthesis, and photosynthesis are shown in more detail. Colors are as described for (A). E = eukaryotic subsystem, B = bacterial subsystem, A = archaeal subsystem.