Figure 1

Set of nine intermediate sequences linking pancreatic lipase with patatin. The accessions of the two starting sequences of FAMILYSEARCHER and the nine intermediates are enclosed in boxes. The large arrows to the left side represent path components found by FAMILYSEARCHER using the respective global starting sequences. The thin arrows between boxes represent directional links found with PSI-BLAST [4] (direction from starting sequence to hit) and are labeled with the corresponding round number of first significant detection (in parentheses) and the inclusion E-value. In addition to the full path, two shortcuts are displayed. The respective alignment regions are (from the top to the bottom, segment boundaries in brackets): AAT95008 [79–249], NP_946830 [63–209], NP_771800 [64–211], YP_049079 [81–177], NP_693664 [158–398], NP_296855 [21–118], NP_799564 [77–124], YP_013380 [8–60] via direct path and [9–119] via shortcut, EAL03661 [423–518] from the YP_013380 side and [397–579] from patatin side. It is important to assure that the connection to EAL03661 and the other intermediates is in the same region regardless of the side of the path (pancreatic lipase or patatin) the search was started. Examining the corresponding PSI-BLAST generated alignments reveals that the boundaries of the alignment starting from the pancreatic side (423 – 518) are completely contained within the alignment starting from the patatin side (397 – 579) of the path.