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Figure 5 | BMC Bioinformatics

Figure 5

From: AtRTPrimer: database for Arabidopsis genome-wide homogeneous and specific RT-PCR primer-pairs

Figure 5

Web interface of AtRTPrimer. Query result and schematic picture of possible amplicons are shown in (A) and (B), respectively. In query result table (A), 'genoppID' is the synonym of AtRTPrimerID and the 'Size' denotes the amplicon length produced by a primer pair. The primer sequences of forward and reverse primer are denoted by 'Fseq' and 'Rseq', respectively. The G or C residues of primer sequences are represented as red colour and the A or T residues as green colour. 'Len' stands for primer length. Here, 'FreeG' are defined as maximal non-specific binding free energy. 'GC' and 'Tm' stand for GC % and melting temperatures of primers. When a user clicks genoppID, a schematic picture shows up as shown in (B). The annotation of a schematic picture is available at Help menu of AtRTPrimer website

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