Figure 2

Cumulative selection of GEPs by sequential application of the functional criteria. Constraints to gene expression resulting from the application of the different criteria. The x-axis represents the fold-change in gene expression with respect to the basal levels for the different genes. The y-axis represents the percentage of GEPs that have a given fold-change in gene expression. Light grey bars represent the distribution of gene expression in GEPs prior to any selection. This distribution is uniform. Green bars represent the distribution of fold change in gene expression in the set of GEPs that obey a given combination of criteria. This distribution is superimposed to the prior distribution, showing how the criteria constrain the expression of different genes. Panels show results of applying several criteria in combination: A - C1-C3,B - C1-C4, C - C1-C5, D - C1-C6, E -C1-C7, and F - C1-C8. Changes in the levels of expression for each gene from the different experimental datasets are represented as discrete points in panel F. Gene expression changes for different genes from the same dataset are shown at the same height in each row of individual plots. Black stars – data from Eisen's experiments at 10 min (BD1 10'); White stars – data from Causton's experiments at 15' (BD2 15'); White circles – data from Gasch's experiments at 10' (DB3 10'); Grey circles – data from Gasch's experiments at 15' (DB3 15'); Black circles – data from Gasch's experiments at 20' (DB3 20').