PYK over-expression and glycerol flux. Box and whisker plots show correlation between trehalose production and changes in the gene expression of PFK and TPS. Each entry in the box-and-whisker plot is represented by a box that spans the distance between two quantiles (0.25 and 0.75) surrounding the median. The median is shown by a horizontal line crossing the green box. "Whiskers" lines extend to span the full range of values for that entry.