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Table 2 Ranges of allowed variations of kinetic parameters

From: Quantitative elementary mode analysis of metabolic pathways: the example of yeast glycolysis

Enzyme Parameter Reference value Minimum Maximum
GLK V max 226.452 150 100000
  K eq 3800 1 100000
  K m·Glc 0.08 0.005 1
  K m·G6P 20 0.02 1000
  K m·ATP 0.15 0.0001 0.3
  K m·ADP 0.23 0.1 1000
PGI V max 339.677 30 100000
  K eq 0.314 0.002 1000
  K m·G6P 1.4 0.001 100
  K m·F6P 0.3 0.001 1000
PFK V max 182.903 50 100000
  g R 5.12 0.5 1000
  L 0 0.66 0.1 50000
  K m·ATP 0.71 0.001 10
  K m·F6P 0.1 0.001 10
  K AMP 0.0995 0.0001 10000
  K ATP 0.65 0.0001 10000
  K F26bP 0.000682 0.0001 10000
  K F16bP 0.111 0.0001 10000
ALD V max 322.258 100 100000
  K eq 0.069 0.0005 10
  K m·F16P 0.3 0.001 50
  K m·GAP 2 0.0001 10
  K m·DHAP 2.4 0.001 100
  K m·GAPi 10 0.02 1000
G3PDH V max 70.15 2 300
  K eq 4300 0.01 100000
  K m·DHAP 0.4 0.001 500
  K m·NADH 0.023 0.001 50
  K m·NAD 0.93 0.001 1000
  K m·Gly 1 0.0001 1000
GAPDH V max 1184.52 200 100000
  K eq 0.045 0.005 10
  K m·GAP 0.21 0.001 2
  K m·BPG 0.0098 0.0005 1000
  K m·NAD 0.09 0.0001 5
  K m·NADH 0.06 0.0005 8
PGK V max 1306.45 30 100000
  K eq 3200 100 100000
  K m·BPG 0.003 0.0001 10
  K m·P3G 0.53 0.001 30
  K m·ADP 0.2 0.002 1000
  K m·ATP 0.3 0.001 30
PGM V max 2525.81 50 100000
  K eq 0.19 0.001 100
  K m·P3G 1.2 0.001 300
  K m·P2G 0.08 0.00002 100
ENO V max 365.806 200 100000
  K eq 6.7 0.01 1000
  K m·P2G 0.04 0.0001 10
  K m·PEP 0.5 0.00003 100
PYK V max 1088.71 300 100000
  K eq 6500 0.1 9000
  K m·PEP 0.14 0.01 9
  K m·Pyr 21 0.1 1000
  K m·ADP 0.53 0.01 4
  K m·ATP 1.5 0.3 100
PDC V max 174.194 100 100000
  K m·Pyr 4.33 0.001 50
ADH V max 810 200 100000
  K eq 6.9·10-5 10-8 0.0005
  K m·Ace 1.11 0.01 1000
  K m·Et 17 0.001 1000
  K m·NADH 0.11 0.001 5
  K m·NADP 0.17 0.001 30