Table 4 Experimental sources for building the interactome. Summary of the experiments used as a foundation for building the interactome, from most frequent (top) to least frequent (bottom). The percentage of the total is listed after each value.
Method | Frequency (%) |
|---|---|
Two hybrid test | 35,759 (69.9) |
Immunoprecipitation | 6,290 (12.3) |
Tandem Affinity Purification (TAP) | 3,503 (6.85) |
Affinity chromatography | 1,070 (2.09) |
Copurification | 572 (1.12) |
Cross-linking | 518 (1.01) |
X-ray crystallography | 511 (1.00) |
In vitro binding | 452 (0.88) |
Biochemical/biophysical | 327 (0.64) |
Gel filtration chromatography | 326 (0.64) |
In vivo kinase activity assay | 185 (0.36) |
Competition binding | 185 (0.36) |
Immunoblotting | 140 (0.27) |
Cosedimentation | 133 (0.26) |
Gel retardation assays | 106 (0.21) |
Native gel electrophoresis | 103 (0.20) |
Other | 973 (1.90) |