Figure 3
From: Biologically relevant effects of mRNA amplification on gene expression profiles
Quantitative real-time PCR (qPCR) of biopsy RNA before and after amplification using a high (cyclophilin A), medium (ribosomal protein S9, RPS9) and low (collagen VI alpha 3, COL6a3) abundance transcript. Material for the 'before amplification' qPCR was obtained from cDNA synthesis from total biopsy RNA; material for the 'after amplification' qPCR was obtained from cDNA synthesis from first round cRNA. After amplification, biopsy #3 yielded aberrant Ct values for all 3 genes; biopsy #5 only for COL6a3.