Figure 5

Reporters that were not detected after amplification ■ had a significantly higher mRNA GC-content (53.7% ± 4.0 vs. 47.8% ± 5.5, P < 0.001) (a), and contained significantly more (b) and longer (c) hairpins as compared to representative control reporters present before and after amplification o (0.76 ± 0.07 vs. 0.38 ± 0.10 hairpins per gene resp., P < 0.01; and 9.4 base pairs (bp) ± 0.3 per hairpin vs. 8.4 bp ± 0.4 resp., P < 0.05. A hairpin must contain 7 or more base pairs. The compared reporters were taken from the same region of expression (same signal intensities).