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Table 6 Correlation of qRT-PCR with Expresso and TM4. We calculate the correlation of qRT-PCR results from the Expresso GP model and the MEV t-test. For each comparison, the analytical result for each gene for which there is a qRT-PCR result for a particular genotype is assembled into a result vector. We used SAS to compute a Pearson correlation of each result vector with the corresponding vector of qRT-PCR results. The computed correlations are as reported above.

From: The statistics of identifying differentially expressed genes in Expresso and TM4: a comparison

 

Genotype-Specific Correlation

Comparison

Col-0

Cvi-0

WS

Th

qRT-PCR versus GP model with MIV input

0.85

0.73

0.66

0.84

qRT-PCR versus GP model with IIVTLSF input

0.83

0.69

0.61

0.78

qRT-PCR versus MEV t-test with IIVTLSF input

0.83

0.65

0.64

0.77