Single Point Mutation Prediction in the 5'UTR ofHCV by RNAMute. A successful prediction by RNAMute, illustrating its potential capability to detect biologically meaningful findings. The G235A point mutation (corresponding to G95A using our indexing scheme) in the 5'UTR of HCV  is predicted by RNAMute to cause a conformational rearrangement. In turn, it is reported to display a dramatic reduction in translation initiation. However, in that reference  based on simple base pairing considerations, it was stated that this mutation alters only the primary sequence. With the availability of RNAMute, alterations in the secondary structure can easily be detected.