Figure 8

Cleavage of a polymer sequence : options and results. This figure illustrates the process involved in cleaving a polymer sequence (a protein) with an enzyme (trypsin). The options for the cleavage are set in the lower left window. Upon cleavage, all the peptides will be generated (those resulting from full sequence cleavage plus the set of peptides corresponding to a single partial cleavage, as required in the options window). The masses of the oligomers (peptides) generated will be computed according to the configuration of the mass calculation engine shown on the upper left window screen capture. The cleavage results are displayed on the right window. When a peptide is clicked (one treeview row), its data are displayed below the list of peptides; if the item is double-clicked, its corresponding sequence is highlighted in the polymer sequence editor window. It is notheworthy that searching for specific masses in the newly generated set of oligomers is allowed by clicking onto the "Find" button.