Figure 3From: Design of microarray probes for virus identification and detection of emerging viruses at the genus levelIn silico validation of the conserved sequence design method. All of the fully sequenced viral genomes (except that of SARS-CoV) in the coronavirus genus were used to determine the conserved sequences. The design method was validated by testing if the computed conserved sequences could successfully detect the novel SARS-CoV. (A) The redundant conserved segments as computed by steps 1 to 4 of the probe design algorithm were reduced to five nonredundant sequence groups by the BLASTN sequence alignment in step 5 of the probe design algorithm. The longest sequence (underlined sequence) in each group was used to represent the group. (B) BLASTN results for the comparison of the group 1 conserved sequence with the SARS-CoV genome. The BLASTN alignment outputs are shown in the figure. The sequence alignment with the SARS-CoV genome shows that the group 1 conserved sequence is located between nucleotides 15732 and 15829 of the SARS-CoV genome. (C) Larger-scale cross-validation of 14 viral genera consisting of 333 nonredundant sets of viral genomes.Back to article page