Mapping rRNA sequence data onto 3D structure of 16S rRNA; Screenshot displaying rRNA sequence data from Desulfovibrio vulgaris superimposed onto the 3D structure of Escherichia coli small subunit rRNA. The rRNA sequence of D.vulgaris is initially aligned in reference to E.coli rRNA sequence and then mapped onto the E.coli 3D structure. Residues in blue represent substitutions or mutations where as regions in red denotes deletions in reference to E.coli rRNA sequence. Green arrows with residues indicate the positions and number of plausible insertions. Part of the structure is shown in more detail in the inset (A). Together with the positional variability maps (Figure 3) specific regions of individual rRNA sequences can be inspected thoroughly residue-by-residue.