Figure 4
From: Epigenetic acquisition of inducibility of type III cytotoxicity in P. aeruginosa
Secretion profiles of various P. aeruginosa strains induced for type III secretion. Coomassie blue-stained SDS-PAGE of TTSS secreted proteins. ExoS and ExoT toxins, the most abundant type III-related exoproducts[3], are indicated on the right side of the gel. IPTG was used at 1 mM. Under corresponding lanes are given the mean values (± SE) of the relative exsA mRNA production compared with the one of PAO1 (set to 1), measured by real time PCR in the same conditions. (A) Lane 1, CHA; lane 2, PAO1; lanes 3 and 4, PAO1 (pexsA ind) without or with a 20 min IPTG pulse; lane 5, PAO1 ExsA-; lanes 6 and 7, PAO1 ExsA- (pexsA ind) without or with a 20-min IPTG pulse; lane 8, PAO1 ExsA- (pexsA ind) with a 20 min IPTG pulse and with IPTG during the calcium depletion. (B) PAO1 (pexsA ind) was pulsed or not with 1 mM IPTG during 20 min then washed with fresh LB. Next, bacteria were maintained in exponential phase of growth by serial dilution with fresh medium during a definite time before calcium depletion as indicated below the gel.