Table 1 PCR primers and probes used, restriction sites are in bold (6-FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine).
From: Epigenetic acquisition of inducibility of type III cytotoxicity in P. aeruginosa
Name | Sequences (5'-3') | Description |
|---|---|---|
EXSAS | CCGAATTC TTATAATATGCAAGGAG | Amplification of exsA |
EXSAR | GGAAGCTT TCAAAAAACGTCAGTTA | Â |
Xbaptaq | CGTCTAGA TTGACAATTCATCGCCTCG | Amplification of exsAind |
KpnlacIq | GGGTACC TCACTGCCCGC TTTCCAGT | Â |
RTExsA | CAGCGAACGCGATGATGCC | Reverse transcription of exsA |
ExsAQS | GAACTGACCGTCCAGGACAT | Amplification and detection of exsA cDNA in RT PCR |
ExsAQR | CAACGCTCGACTTCACTCAA | Â |
ExsADL | (6-Fam)CCTGGCGAGTTGCTTTTCGTCCGC(Tamra) | Â |
16s sens | AAGCAACGCGAAGAACCTTA | Amplification and detection of 16S rRNA cDNA in RT PCR |
16as | CACCGGCAGTCTCCTTAGAG | Â |
16sDL | (6-Fam)ACGAAGGGACGCACGAG(Tamra) | Â |