Figure 5

Demonstration of successful clustering of the pathogen Vibrio cholerae among 477 members of the genus Vibrio by mass spectrometric observables. The presented Neighbor-joining (NJ) tree is based on mass-spectral coincidence analysis of the Lane-AB 16S rDNA sequence region of the genus Vibrio. Only the names of 20 strains of the pathogen V.cholerae are displayed out of 477 amplifiable Vibrio species or strains were used for generation of the NJ tree. The mass spectrometric observable distances from near-neighbors presented may be used to determine the limit of discrimination for a given pathogen and base-specific cleavage reaction.