Figure 7
From: A methodology for global validation of microarray experiments
Validation of microarray experiments by qrPCR (data averaged across experiments; top-ranked and random-stratified data combined). Data in (A) were combined from Figures 5A and B; (B) Same as in (A) with log2 FCs < 0.5 (yellow +'s) removed. (C-D) Standardized (z-scores) data from A-B. (E) Pair-link diagram of data from panel A; apparent regression away from the mean is evident by the mostly positive line slopes, especially among the larger microarray FCs. (F) Pair-link diagram of data from panel C; regression toward the mean is evident by the tendency of the lines to cross. Blue data points in all graphs are from the top-ranked sampling method; orange points are from the random-stratified method; the single red point was included in both samples. The regression lines are: the identity line (red), least-squares (blue) and loess (yellow).