Table 1 Reactions for scaffold with 3 binding sites. A complete mechanistic model of a scaffold protein with 3 binding domains (1,2,3), where each domain can bind one effector protein (E₁, E₂, E₃), has to consider the following 12 reactions. The kinetic parameters for each reaction can be denoted with k_+ifor the association and k_-ifor the dissociation reaction

R[0, 0, 0]+E₁ ⇋ R[1, 0, 0]     (49a)

R[0, 0, 0]+E₂ ⇋ R[0, 1, 0]     (50a)

R[0, 0, 0]+E₃ ⇋ R[0, 0, 1]     (51a)

R[0, 1, 0]+E₁ ⇋ R[1, 1, 0]     (49b)

R[0, 0, 1]+E₂ ⇋ R[0, 1, 1]     (50b)

R[0, 1, 0]+E₃ ⇋ R[0, 1, 1]     (51b)

R[0, 0, 1]+E₁ ⇋ R[1, 0, 1]     (49c)

R[1, 0, 0]+E₂ ⇋ R[1, 1, 0]     (50c)

R[1, 0, 0]+E₃ ⇋ R[1, 0, 1]     (51c)

R[0, 1, 1]+E₁ ⇋ R[1, 1, 1]     (49d)

R[1, 0, 1]+E₂ ⇋ R[1, 1, 1]     (50d)

R[1, 1, 0]+E₃ ⇋ R[1, 1, 1]     (51d)