Skip to main content
Figure 3 | BMC Bioinformatics

Figure 3

From: PIPE: a protein-protein interaction prediction engine based on the re-occurring short polypeptide sequences between known interacting protein pairs

Figure 3

Novel protein-protein interactions. PIPE can successfully detect novel protein-protein interactions. With a score of 136 in (a), PIPE analysis predicts an interaction between YGL227W and YMR135C. (b) TAP-tag analysis confirms the interaction between YGL227W and YMR135C. When YGL227W is TAP-tagged, YMR135C is purified as an interacting subunit (panel 1). Reciprocal TAP-tagging of YMR135C also identifies YGL227W as an interacting partner, confirming the validity of the observed interaction between the two proteins (panel 2). Panels 3 and 4 show the purifications of TAP-tagged YGL227W strains in which either YDR255C (YDR255CΔ) or YMR135C (YMR135CΔ) were deleted, respectively. Deletion of YDR255C (panel 3) had no effect in the co-purification of other subunits. However, when YMR135C (panel 4) was deleted, the interactions between TAP-tagged YGL227W and most other subunits were eliminated. Panel 5 is used as a control and shows the purification of a strain, which is not tagged.

Back to article page

BMC Bioinformatics

ISSN: 1471-2105

Contact us