Results for patient 2. Mean and standard deviation of the computed relative mRNA proportions for macrophages (macro), fibroblasts (fibro) and non-adherent cells (nadc) for patient 2 as a function of the number k of genes included in the trimmed regression approach (Methods section, subsection Mathematical model). The measured expression profiles are MAS-S probe set summaries normalized by a symmetric trimmed mean (t) (Methods section, subsection Data preparation). The reconstituted tissue profile is either not normalized (dashed) or normalized by stepwise local regression (lr, solid) (Methods section, Data preparation). The proportions are determined from the respective means at the number of genes for which the standard deviations approach zero. The chosen numbers are indicated by the dashed and solid (lr) vertical lines, respectively. The convergence is faster for additional local regression (lr), but the resulting proportions are similar. The mRNA proportions are estimated to be p
= 0.82/0.79 (lr) for macrophages, p
= 0.09/0.06 (lr) for fibroblasts and p
= 0.10/0.15 (lr) for non-adherent cells. These values were determined at 6000 and 5000 (lr) included genes, respectively.