1) calculate the means and standard deviations of the computed mRNA proportions as a function of the number of included genes: |
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□ increase the number of included genes k |
• generate l random starting values {} j = 1,..., l, in which = () |
○ determine p j = (p Mj , p Fj , p Nj ) by minimizing the objective function starting from |
○ store p j |
• calculate the mean and standard deviation of {p j }j = 1,...,l for the current value of k |
2) determine the means of the computed mRNA proportions at the smallest number of included genes, for which the standard deviations approach zero. |