Skip to main content
Figure 4 | BMC Bioinformatics

Figure 4

From: Comprehensive quality control utilizing the prehybridization third-dye image leads to accurate gene expression measurements by cDNA microarrays

Figure 4

Quality dependent data filtering and normalization. Data are from one hybridization between day 65 and day 40 DP rats of experiment 1. (A) Log ratio distribution before normalization is plotted against q com . Spots with q com < 0.20 exhibit significantly increased variability, and will be reset to q com = 0. Normalization will be performed for all spots with q com > 0.20. Also shown are the normalization factor (solid line) and the local 3 SD from mean (dotted line). (B) The same data after filtering and normalization.

Back to article page

BMC Bioinformatics

ISSN: 1471-2105

Contact us