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Figure 1 | BMC Bioinformatics

Figure 1

From: Development of an open source laboratory information management system for 2-D gel electrophoresis-based proteomics workflow

Figure 1

A general procedure in 2-D PAGE-based proteomics workflow. After separation of proteins by 2-D PAGE, we acquire 2-D gel images using a laser-scanning imager system and analyze the gel images with the expert software in the 2-D PAGE-based proteomics workflow. And we operate in-gel digestions using the expert machine after the spot cutting of 2-D PAGE gel. We analyze the digested peptide masses and search database for protein identification using the search tool of MS-fit (The Regents of the University of California) [15] or Mascot (Matrix Science Ltd) following Mass Spectrometric analysis using the expert equipment.

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