Protein kinases associated with the yeast phosphoproteome

Table 3 Predicted protein kinase- phosphoprotein pairs supported by experimental evidence

Substrate protein	Predicted protein kinase	Phosphopeptide *	Scansite score **	Estimated probability ***
Yak1p	Tpk1p	R RKSS LVV	0.145, 0.02	0.6, 0.95
Yak1p	Tpk1p	R RAS LNS	0.127	0.75
Sra1p	Tpk1p	RSRS SVM	0.103	0.8
Acc1p	Snf1p	R AVS VSD	0.145	0.6
Pfk2p	Snf1p	K VHS YTD	0.145	0.6
Hog1p	Ste7p	PQMT GY VST	0.19	0.33
Bni5p	Cdc28p	PVSS PIT	0.145	0.6
Gpd1p	Bck1p	R SSS SVS LKA	0.141	0.6
Hsp26p	Ume5p	LANT PAK	0.082	0.85
Msn2p	Pkc1p	R RPS YRR	0.147	0.6
Sok2p	Yak1p	K SIS PRT	0.148	0.6
Ssd1p	Hog1p	SLSS PTK	0.039	0.95
Ste2p	Fus3p	QLPT PTS SKN	0.04	0.99

* The phosphorylated residues are underlined, and the residues not present in the phosphopeptide sequences [4] are shown in italic. When there is more than one phosphorylation site, the one discussed is shown in bold, unless the same protein kinase is predicted for all sites in the peptide.
** Scansite [11] scores were calculated as described in the Methods section. When the same protein kinase is predicted for all sites in the peptide, the scores are given for the respective sites, starting at the N-terminus. If more than one protein kinase yields a similar score, all the possible kinases are listed.
*** Probabilities were calculated as described in the Methods section (Figure 1). When the same protein kinase is predicted for all sites in the peptide, the values are given for the respective sites, starting at the N-terminus.