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Table 4 Selection of cDNAs classified as "bad_multiple_exon_cdna" by CAFTAN.

From: CAFTAN: a tool for fast mapping, and quality assessment of cDNAs

 

cDNA_Name

Signal

VEGA_matcht

VEGA match

Problem

1

OTTHUMT00000002088

No signal

perfect

TNFRSF14-001

The sequence ends with the poly A signal AAUAUA

2

OTTHUMT00000002210

No signal

perfect

SLC35E2-001

Partial Sequence in the 3' UTR end. The sequence is longer and for that reason is not possible to find the polyA signal

3

OTTHUMT00000003581

No signal

perfect

PARK7-005

Partial Sequence in the 3' UTR end. The sequence is longer and for that reason is not possible to find the polyA

4

OTTHUMT00000003582

No signal

perfect

PARK7-006

RPL20 Gen, eventually (A)ATGAA(A) signal

5

OTTHUMT00000004064

No signal

perfect

C1orf86-001

Not a perfect cDNA, the last exon fails and for that reason it is not possible to find a polyA signal

6

OTTHUMT00000004201

No signal

perfect

PHF13-001

False 3' end (artefact), the real 3' end is upstream from this point and is supported by many ESTs and a canonical polyA signal

7

OTTHUMT00000005013

No signal

perfect

CTNNBIP1-002

Good cDNA, this cDNA does not have canonical or typical non-canocial polyA signals checked in CAFTAN. Putative polyA signal: ATGTAAATAT

8

OTTHUMT00000005014

No signal

perfect

CTNNBIP1-003

The real 3' UTR is a little bit longer and contains a canonical polyA signal 20 bp upstream from the polyA tail.

9

OTTHUMT00000005015

No signal

perfect

CTNNBIP1-004

Partial Sequence in the 3' UTR end, fail the terminal bases, which make a perfect canonical polyA signal (AAUAAA) 15 bp upstream from the polyA tail

10

OTTHUMT00000005017

No signal

perfect

UBE4B-002

Partial Sequence in the 3' UTR end, fail many terminal bases, which make a perfect canonical polyA signal (AAUAAA)

11

OTTHUMT00000005070

No signal

perfect

SDF4-002

Partial Sequence in the 3' UTR end, fail almost all the 3 'UTR

12

OTTHUMT00000005103

No signal

perfect

KIF1B-002

Partial Sequence in the 3' UTR end, fail almost all the 3 'UTR

13

OTTHUMT00000005105

No signal

perfect

KIF1B-004

Partial Sequence in the 3' UTR end, fail almost all the 3 'UTR

14

OTTHUMT00000005430

No signal

perfect

UBE2J2-001

Partial Sequence in the 3' UTR end, fail almost all the 3 'UTR

  1. All of them lack the presence of a polyA signal but have a perfect match to VEGA transcripts, as it should be expected. The manual inspection by an expert curator of these VEGA CDNAs showed that all of them but 2 (No. 4 and No. 8) where not complete failing part of the 3'UTR region or having artifact 3' end like No. 6.