Normalization and expression changes in predefined sets of proteins using 2D gel electrophoresis: A proteomic study of L-DOPA induced dyskinesia in an animal model of Parkinson's disease using DIGE

Table 1 2D-DIGE experimental design

Each gel had three samples. Two corresponding to a sample of interest (labeled with Cy3 or Cy5) and a pooled standard which is a mix of all samples used in the study (labeled with Cy2). The samples were from four different groups of animals: untreated animals (Ctl), those treated with Mptp (Mptp), those treated with Mptp plus one acute dose of L-DOPA (Ldopa) and finally those treated with Mptp and long-term L-DOPA treatment (Dysk).

ISSN: 1471-2105