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Table 1 Target protein ranking among the search hit results using precursor ion m/z values, product ion m/z values, and/or LC-ETs.

From: HybGFS: a hybrid method for genome-fingerprint scanning

Gene Product

Rank

 

Precursor

Precursor and ET

Precursor and Product

All

Mascot

Average

157.83

35.77

4.69

1.85

-

PolA

1

1

1

1

1

AdhE

1

1

1

1

1

PykF

2

1

1

1

1

AceE

7

1

1

1

1

FumA

9

1

1

1

1

AcnA

9

1

1

1

1

Pgk

10

1

1

1

1

Eno

12

1

1

1

1

GapA

16

1

1

1

1

Ppc

21

1

1

1

1

SucC

25

1

1

1

1

Pgi

30

1

1

1

1

PtsI

5

2

1

1

1

RpiA

10

2

1

1

1

SucB

6

3

1

1

1

PykA

8

3

1

1

1

FumB

10

3

1

1

1

IcdA

11

3

1

1

1

Gnd

28

3

1

1

1

AceA

301

3

1

1

1

LpdA

28

4

1

1

1

Fba

282

6

1

1

1

Zwf

55

7

1

1

1

FumC

211

7

1

1

1

SucD

324

7

1

1

1

TalA

155

8

1

1

1

SfcA

56

9

1

1

1

SucA

34

11

1

1

1

Fbp

512

11

1

1

1

GpmA

34

12

1

1

1

Acs

111

14

1

1

1

PflA

139

14

1

1

1

LdhA

207

27

1

1

1

PckA

325

31

1

1

1

TalB

493

32

1

1

N.I.

AckA

617

50

1

1

1

Edd

139

80

1

1

4

GpmB

1752

767

1

1

3

PoxB

19

2

2

1

1

Mdh

62

4

2

1

1

PflB

4

1

4

1

1

PpsA

27

8

4

1

2

SdhA

294

271

7

1

1

GltA

129

1

16

1

1

AceB

151

13

16

1

1

Eda

312

15

23

7

N.I.

PfkA

429

15

77

32

N.I.

  1. For comparison, the results searched using Mascot software are also shown. Each recombinant protein's name we experimentally tested in the validation dataset is listed, as is its ranking in the search results.