Table 3 The places of the model.

pheromone released by an MATα cell in the surroundings

mating pheromone receptor of the modelled MATa cell

complex consisting of the α-factor and the Ste2 receptor

the above named complex is activated by a conformation change

heterotrimeric G protein, which is coupled to the Ste2 receptor

dissociated Gα subunit (exchange of GDP to GTP in this monomer)

G-protein Gβγ subunits in a dimeric form

Cdc24, i.e., guanine nucleotide exchange factor of Cdc42

Cdc42 located at the plasma membrane

protein kinase Ste20

Ste5, acting as a scaffold protein

protein complex consisting of Ste5 and Ste11

MAP kinase Fus3

protein complex consisting of Ste7 and Fus3

MAPK complex consisting of Ste5, Ste11, Ste7 and Fus3

Ste20 located at the plasma membrane, i.e., near the MAPK complex

as complex1, but Ste11 is activated additionally

as complex2, but Ste7 is activated additionally

as complex3, but Fus3 is activated additionally

dissociated Fus3 in the activated form

as complex4, but without Fus3

complex containing Ste12 repressed by Fus3 or Kss1 and Dig1/Dig2

Ste12 inhibitors, i.e., cofactors for the repression

Ste12 released out of the repression complex

activated transcription factor Ste12

phosphatase Msg5 being able to deactivate Fus3 or Kss1

deactivated Fus3

pheromone regulated genes encoding mating related cell responses

synthesised protease Bar1 located in the nucleus

Bar1 secreted in the cell environment

synthesised Far1 located in the nucleus in an inactive form

Far1 activated by phosphorylation

active Far1 located in the cytosol

synthesised Sst2 located in the nucleus in an inactive form

Sst2 activated by phosphorylation

active Sst2 located in the cytosol

complex labelled for degradation by phosphorylation

MAP kinase Kss1 activated by phosphorylation

inactive Kss1

protein Akr1 located at plasma membrane

kinases Yck1/Yck2 being able to label the Ste2 for degradation

receptor labelled for ubiquitination and endocytosis