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Figure 2 | BMC Bioinformatics

Figure 2

From: Statistical analysis of an RNA titration series evaluates microarray precision and sensitivity on a whole-array basis

Figure 2

Selected MA plots for the titration series. M (log2 transformed fold change value) was plotted versus A (total intensity for that spot) for all genes on the arrays. Columns represent Spotted cDNA, Spotted oligonucleotide, Agilent and Affymetrix. The top row shows the A1 sample (MCF7) vs Jurkat, the second row shows the A3 mixture (88% MCF7, 12% Jurkat) vs Jurkat, the third row shows A5 (50% MCF7, 50% Jurkat) vs Jurkat and the bottom row is A6 (100% Jurkat) in a self-self hybridization. As expected the A1 sample shows the most differential expression in the series of arrays. Similarly, the A6 sample shows very little differential expression. The green line represents the theoretical minimum fold change. No elements should report a fold change below this line.

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