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Table 1 Mixing proportions used for titration series

From: Statistical analysis of an RNA titration series evaluates microarray precision and sensitivity on a whole-array basis

Sample

MCF7 c

Jurkat 1-c

A1

100%

0%

A2

94%

6%

A3

88%

12%

A4

76%

24%

A5

50%

50%

A6

0%

100%

B

0%

100%

  1. Samples A1 through A6 were created by mixing total mRNA from MCF7 breast epithelial cells with mRNA derived from the Jurkat T cell line in the proportions shown. The sample B was used as a reference RNA in all two-color hybridizations (spotted cDNA, spotted oligonucleotide and Agilent). For Affymetrix hybridizations, samples A1 through B were hybridized independently.