Table 1 Non-redundant significant features meeting the criteria (P _wald < 0.01) and (P ₇₀ < 0.01 or P ₉₀ < 0.01)

2nd nucleotide = A

12.1

16.0

33.8

38.1

0.01

0.0026

0.0019

4th nucleotide = C

14.1

15.4

31.5

39.0

0.098

0.00036

0.0075

6th nucleotide ≠ C

14.3

15.6

35.0

35.1

0.00066

0.0089

0.0052

7th nucleotide ≠ U

14.4

15.9

34.5

35.2

0.01

0.0043

0.0091

9th nucleotide = C

11.1

16.6

32.6

39.6

0.008

0.00021

0.00053

17th nucleotide = A

11.4

15.5

37.1

35.9

0.00049

0.1

0.0049

18th nucleotide ≠ C

14.4

15.9

34.3

35.4

0.01

0.00071

0.0048

19th nucleotide = (A/U)

12.0

16.0

35.3

36.7

0.00029

0.0043

0.000058

At least three (A/U)s in the seven nucleotides at the 3' end

13.4

16.4

33.7

36.5

0.00001

0.00001

2.5E-09

No occurrences of four or more identical nucleotides in a row

14.2

15.9

35.4

34.5

0.00001

0.012

0.0014

No occurrences of G/C stretches of length 7 or longer

14.3

16.4

34.9

34.4

0.00001

0.00001

0.000015

G/C content is between 35% and 60%

13.3

16.7

35.1

35.0

0.00001

0.0019

0.00018

T_m is between 20 and 60°C

13.2

16.5

35.0

35.3

0.0045

0.023

0.003

Binding energy of N16–N19 > -9 KCal/Mol

11.8

17.1

34.0

37.1

0.01

0.0026

0.00025

Binding energy of N16–N19 – binding energy of N1–N4 is between 0 and 1 KCal/Mol

12.6

14.9

32.5

39.9

0.01

0.00036

0.0078

Local folding potential (mean) ≥ -22.72 KCal/Mol

12.0

14.6

34.7

38.7

0.00001

0.00001

9.3E-09

Target site is on CDS

14.4

16.2

34.3

35.2

0.00001

0.00001

0.000055

Cell line = HeLa

7.9

10.6

41.2

40.3

0.00001

0.00016

4.0E-09

Test method = Western blot

10.8

15.5

34.8

36.9

0.00001

0.00001

3.8E-14

Test object ≠ mRNA

13.1

14.5

34.8

37.6

0.00001

0.00001

9.3E-10