Table 3 Correlation of thermodynamic stability difference of the 5'/3' ends of siRNA duplexes with siRNA activity (AS) and with the ratio between the antisense strand and sense strand activities (AS/SS). Differences in the stability of the 5' and 3' ends were calculated for 2 (Diff2), 3 (Diff3), 4 (Diff4) and 5 (Diff5) terminal nucleotides in siRNA duplexes.
From: Computational models with thermodynamic and composition features improve siRNA design
Data set | Activity or ratio | Correlation coefficients | Reference | |||
|---|---|---|---|---|---|---|
| Â | Â | Diff2 | Diff3 | Diff4 | Diff5 | Â |
28 siRNAs | AS | -0.245 | -0.196 | -0.171 | -0.103 | Hu et al. [11] |
28 siRNAs | AS/SS | -0.384 | -0.356 | -0.368 | -0.278 | Hu et al. [11] |
18 siRNAs | AS | -0.870 | -0.869 | -0.748 | -0.767 | Schwartz et al. [3] |
18 siRNAs | AS/SS | -0.629 | -0.544 | -0.471 | -0.453 | Schwartz et al. [3] |
653 siRNAs | AS | -0.356 | -0.332 | -0.292 | -0.228 | This work |