Table 1 Options for the pop0ligo.exe and uniq0ligo.exe executables
From: OligoSpawn: a software tool for the design of overgo probes from large unigene datasets
| Â | flag | description | default |
|---|---|---|---|
pop0ligo.exe | -c <cores color> | minimuin number of unigenes in which a overgo's core must occur T c (must be ≥ 2) | 5 |
| Â | -g <GC%> | minimum GC% | 45% |
| Â | -G <GC%> | maximum GC% | 55% |
|  | -m <temperature> | minimum melting temperature | 0°C |
|  | -M <temperature> | maximum melting temperature | 100°C |
| Â | -r <file.fasta> | fasta file containing the repeat database | Â |
uniqOligo.exe | -g <GC%> | minimum GC% | 45% |
| Â | -G <GC%> | maximum GC% | 55% |
|  | -m <temperature> | minimum melting temperature | 0°C |
|  | -M <temperature> | maximum melting temperature | 100°C |
| Â | -r <file.fasta> | fasta file containing the repeat database | Â |
| Â | -t <number> | number of overgos selected for each unigene | 1 |