Figure 8

An interaction map of Vaccinia virus proteins. The protein-protein interaction data in Biozon can be viewed as a subgraph, with many interconnected elements. From this graph we compiled the set of all connected components, and each component was embedded in a two-dimensional Euclidean space, using the algorithm of 48 with the graph distances as input. The map shown is a subnetwork of Vaccinia virus proteins that seem to control its activity through a series of mediated interactions or by forming a complex. For example, the inactivation of protein G2 (docID 507266) renders the virus dependent upon isatin-beta-thiosemicarbazone for growth. This protein interacts with Envelope protein H5 (docID 465934) that interacts with protein A49 (840436) whose function is unknown, as well with Viral DNA polymerase processivity factor. The latter interacts with UDG (Uracil-DNA glycosylase docID 502617), as well as with protein D5 (Putative DNA replication factor). Proteins that directly interact are positioned closely in this map, while proteins that are connected through mediated interactions are positioned farther apart. The set of 7 proteins in this connected component form an interesting subgraph that was exposed with the embedding algorithm.