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Table 1 The sample real-time PCR data for analysis. In this data set, there two types of samples (treatment and control); two genes (reference and target); and four concentrations of each combination of gene and sample. For data quality control and ANCOVA analysis, the real-time PCR sample data set can be grouped in four groups according to the combination of sample and gene. The Control-Target combination effect was named group 1, Treatment-Target group 2, Control-Reference group 3 and Treatment-Reference group 4.

From: Statistical analysis of real-time PCR data

Replicate Sample Gene Concentration Ct Group (Class)
1 Control Target 10 23.1102 1
2 Control Target 10 22.9003 1
3 Control Target 10 22.8972 1
1 Control Target 2 26.5801 1
2 Control Target 2 26.2139 1
3 Control Target 2 26.0606 1
1 Control Target 0.4 28.1125 1
2 Control Target 0.4 28.1899 1
3 Control Target 0.4 27.5949 1
1 Control Target 0.08 30.2772 1
2 Control Target 0.08 30.4667 1
3 Control Target 0.08 30.7571 1
1 Treatment Target 10 21.7813 2
2 Treatment Target 10 21.7564 2
3 Treatment Target 10 21.641 2
1 Treatment Target 2 23.7965 2
2 Treatment Target 2 23.7571 2
3 Treatment Target 2 23.724 2
1 Treatment Target 0.4 26.3794 2
2 Treatment Target 0.4 26.2542 2
3 Treatment Target 0.4 25.9621 2
1 Treatment Target 0.08 28.5479 2
2 Treatment Target 0.08 28.3894 2
3 Treatment Target 0.08 28.3416 2
1 Control Reference 10 19.7415 3
2 Control Reference 10 19.494 3
3 Control Reference 10 19.3906 3
1 Control Reference 2 21.9838 3
2 Control Reference 2 22.4435 3
3 Control Reference 2 22.57 3
1 Control Reference 0.4 24.8109 3
2 Control Reference 0.4 24.4327 3
3 Control Reference 0.4 24.2342 3
1 Control Reference 0.08 26.7319 3
2 Control Reference 0.08 26.8206 3
3 Control Reference 0.08 26.822 3
1 Treatment Reference 10 18.4468 4
2 Treatment Reference 10 18.8227 4
3 Treatment Reference 10 18.3061 4
1 Treatment Reference 2 21.2568 4
2 Treatment Reference 2 21.0956 4
3 Treatment Reference 2 20.8473 4
1 Treatment Reference 0.4 23.2322 4
2 Treatment Reference 0.4 22.9577 4
3 Treatment Reference 0.4 23.2415 4
1 Treatment Reference 0.08 25.4817 4
2 Treatment Reference 0.08 25.608 4
3 Treatment Reference 0.08 25.5675 4