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Table 2 ΔCt calculation. The table presents the calculation of ΔCt, which is derived from subtracting Ct number of reference gene from that of the target gene. Con stands for concentration.

From: Statistical analysis of real-time PCR data

Sample Gene Con Ct Sample Gene Con Ct ΔCt
Control Target 10 23.1102 Control Reference 10 19.7415 3.3687
Control Target 10 22.9003 Control Reference 10 19.494 3.4063
Control Target 10 22.8972 Control Reference 10 19.3906 3.5066
Control Target 2 26.5801 Control Reference 2 21.9838 4.5963
Control Target 2 26.2139 Control Reference 2 22.4435 3.7704
Control Target 2 26.0606 Control Reference 2 22.57 3.4906
Control Target 0.4 28.1125 Control Reference 0.4 24.8109 3.3016
Control Target 0.4 28.1899 Control Reference 0.4 24.4327 3.7572
Control Target 0.4 27.5949 Control Reference 0.4 24.2342 3.3607
Control Target 0.08 30.2772 Control Reference 0.08 26.7319 3.5453
Control Target 0.08 30.4667 Control Reference 0.08 26.8206 3.6461
Control Target 0.08 30.7571 Control Reference 0.08 26.822 3.9351
Treatment Target 10 21.7813 Treatment Reference 10 18.4468 3.3345
Treatment Target 10 21.7564 Treatment Reference 10 18.8227 2.9337
Treatment Target 10 21.641 Treatment Reference 10 18.3061 3.3349
Treatment Target 2 23.7965 Treatment Reference 2 21.2568 2.5397
Treatment Target 2 23.7571 Treatment Reference 2 21.0956 2.6615
Treatment Target 2 23.724 Treatment Reference 2 20.8473 2.8767
Treatment Target 0.4 26.3794 Treatment Reference 0.4 23.2322 3.1472
Treatment Target 0.4 26.2542 Treatment Reference 0.4 22.9577 3.2965
Treatment Target 0.4 25.9621 Treatment Reference 0.4 23.2415 2.7206
Treatment Target 0.08 28.5479 Treatment Reference 0.08 25.4817 3.0662
Treatment Target 0.08 28.3894 Treatment Reference 0.08 25.608 2.7814
Treatment Target 0.08 28.3416 Treatment Reference 0.08 25.5675 2.7741