Typical images from the 2-D HeLa cell image collection used in this study. Images are shown for cells labelled with antibodies against an ER protein (A), the Golgi protein giantin (B), the Golgi protein GPP130 (C), the lysosomal protein LAMP2 (D), a mitochondrial protein (E), the nucleolar protein nucleolin (F), transferring receptor (H), and the cytoskeletal protein tubulin (J). Images are also shown for filamentous actin labelled with rhodamine-phalloidin (G) and DNA labelled with DAPI (K). Scale bar = 10 μm. From .