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Figure 2 | BMC Bioinformatics

Figure 2

From: Microarray image analysis: background estimation using quantile and morphological filters

Figure 2

Variability of background pixels as a function of intensity. The median absolute deviation versus the median background pixel intensity in the green (left) and the red (right) channels on Slide 1 calculated using a window with a radius of 35 pixels (spot pixels excluded). There is a linear relationship between the median absolute deviation and the signal, and the scale factor is larger in the red channel (slope 0.19) compared to the green channel (0.11). For normal distributions, the standard deviation is 1.4826 times the median absolute deviation.

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