Morphological background as a function of structuring element size. The mean of Spot's morph.close.open (δ
) as a function of k
taken over all 7680 spots in the red channel on Slide 1. The size of the structuring element B is (kscales
) and the size of B' is (kscale(s
- 2) × kscale(s
- 2)), where s
are the spot-separating distances by row and column, respectively. The default value kscale = 2.5 was used, which for Slide 1 to 4 gives a structuring element (B) of size 62 × 62.