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Figure 3 | BMC Bioinformatics

Figure 3

From: Improvement in the Reproducibility and Accuracy of DNA Microarray Quantification by Optimizing Hybridization Conditions

Figure 3

Signal-to-background ratios under different hybridization or washing conditions. A. GlassHyb™ buffer and washing condition 1. B. GlassHyb™ with more stringent washing condition 2. C. 5× SSC, 0.1% SDS and 32% formamide buffer with washing condition 1. Oligo, rat-specific probe; Lambda, bacteriophage lambda-specific probe; Blank, no probe printed. Replicate experiments were conducted and the correlation coefficients were 0.94, 0.97, and 0.97 for conditions shown in panels A, B, and C, respectively.

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BMC Bioinformatics

ISSN: 1471-2105

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