Single antibody, different blockade signals. (A) The lower-level (LL) blockade of this signal is not as deep as the others. (B) The LL blockade of this signal is about mid-way between those shown. (C) The LL blockade of this signal is the deepest, and difficult to eject with voltage reversal (requiring several attempts). The three signal classes represented by the above, as well as other, less common blockade signals (not shown), correspond to nanopore characterization of different epitopes of the antibody.